Amperometric L-Lactate Biosensor Based upon a Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Modified Screen-Printed Graphite Electrode

This work describes a novel L-lactate biosensor based on the immobilization of dehydrogenase enzyme screen-printed electrode modified with ternary composite gold nanoparticles, electrochemically-reduced graphene oxide, and poly (allylamine hydrochloride). The was stabilized by crosslinking glutaraldehyde. Applied working potential, pH NAD+ concentration were optimized. reports specific sensitivity 1.08 µA/mM·cm2 in range up to 3 mM L-lactic acid detection limit 1 µM. operational long-term stability as well good selectivity allowed measurement dairy products wine samples.